Journal: Journal of Experimental & Clinical Cancer Research : CR
Article Title: C-terminal binding protein-2 triggers CYR61-induced metastatic dissemination of osteosarcoma in a non-hypoxic microenvironment
doi: 10.1186/s13046-025-03350-6
Figure Lengend Snippet: CtBP2 favors cell migration. A Representative images of K7M2 cell migration after 10 h incubation, as assessed by Boyden chamber assay. CtBP2-silenced K7M2 cells were stained in green, and CtBP2-overexpressing cells were stained in red before seeding (bar = 100 µm). B Estimation plot representing the relative number of migrating cells. The difference between the group means is represented on the right. C Representative images of cell migration (wound healing assay) taken at time 18 h after the wound. Control and CtBP2-silenced K7M2 cells were incubated in the presence or absence of recombinant CYR61 (1 µg/mL). The dotted rectangles outline the initial wound surface. D Quantitative evaluation of the cell migration rate. Results are expressed as box plot ( n = 15–22). An asterisk (*) indicates a statistically significant difference ( p < 0.05 vs. Control)
Article Snippet: The cell migration rate was evaluated using modified Boyden chamber (8 μm pore size Falcon® Transwell inserts, Corning, Boulogne-Billancourt, France).
Techniques: Migration, Incubation, Boyden Chamber Assay, Staining, Wound Healing Assay, Control, Recombinant